The Promotion Playoff was played by the teams placed 1st and 2nd of each zone. The winning team was declared champion and was automatically promoted to Primera División. The teams that lost in semifinal joined into the Quarterfinals of the Second Promotion Playoff, and the team that lost in the final joined in the semifinal of the Second Promotion Playoff.
Note: Banfield won 6–3 on aggregate, promoting to Primera División
Second Promotion Playoff
The Second Promotion Playoff or Torneo Reducido was played by the teams placed 3rd to 6th of each zone. Instituto and Gimnasia y Esgrima (CdU) joined in the Quarterfinals, and Quilmes joined in the Semifinals. The winner was promoted to Primera División.
Note: Nueva Chicago won 4–2 on aggregate, promoting to Primera División.
Promotion Playoff Primera División-Primera B Nacional
The best two teams of each zone that were not promoted (Quilmes and Instituto) played against the 18th and the 17th placed of the Relegation Table of 2000–01 Primera División.
Argentinos Juniors remains in Primera División after a 1-1 aggregate tie by virtue of a "sports advantage". In case of a tie in goals, the team from the Primera División gets to stay in it.
Belgrano remains in Primera División after a 1-1 aggregate tie by virtue of a "sports advantage". In case of a tie in goals, the team from the Primera División gets to stay in it.
Relegation
7 teams were relegated: 2 teams with the worst co-efficient from Interior Zone, 2 teams with the worst co-efficient from Metropolitana Zone and 3 more teams regardless their affiliation.