Aphidicolan-16beta-ol synthase

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Aphidicolan-16β-ol synthase
Aphidicolan-16β-ol synthase
Identifiers
EC no.	4.2.3.42
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
PMC
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PMC	articles
PubMed	articles
NCBI	proteins

Aphidicolan-16β-ol synthase (EC 4.2.3.42, PbACS) is an enzyme with systematic name 9α-copalyl-diphosphate diphosphate-lyase (aphidicolan-16β-ol-forming).^[1]^[2] This enzyme catalyses the following chemical reaction

9α-copalyl diphosphate + H₂O

\rightleftharpoons

aphidicolan-16β-ol + diphosphate

This is a bifunctional enzyme, which also has EC 5.5.1.14 activity.

References

^ Oikawa H, Toyomasu T, Toshima H, Ohashi S, Kawaide H, Kamiya Y, Ohtsuka M, Shinoda S, Mitsuhashi W, Sassa T (May 2001). "Cloning and functional expression of cDNA encoding aphidicolan-16 β-ol synthase: a key enzyme responsible for formation of an unusual diterpene skeleton in biosynthesis of aphidicolin". Journal of the American Chemical Society. 123 (21): 5154–5. doi:10.1021/ja015747j. PMID 11457369.
^ Toyomasu T, Nakaminami K, Toshima H, Mie T, Watanabe K, Ito H, Matsui H, Mitsuhashi W, Sassa T, Oikawa H (January 2004). "Cloning of a gene cluster responsible for the biosynthesis of diterpene aphidicolin, a specific inhibitor of DNA polymerase α". Bioscience, Biotechnology, and Biochemistry. 68 (1): 146–52. doi:10.1271/bbb.68.146. PMID 14745177.

External links

Aphidicolan-16beta-ol+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

[1] Oikawa H, Toyomasu T, Toshima H, Ohashi S, Kawaide H, Kamiya Y, Ohtsuka M, Shinoda S, Mitsuhashi W, Sassa T (May 2001). "Cloning and functional expression of cDNA encoding aphidicolan-16 β-ol synthase: a key enzyme responsible for formation of an unusual diterpene skeleton in biosynthesis of aphidicolin". Journal of the American Chemical Society. 123 (21): 5154–5. doi:10.1021/ja015747j. PMID 11457369.

[2] Toyomasu T, Nakaminami K, Toshima H, Mie T, Watanabe K, Ito H, Matsui H, Mitsuhashi W, Sassa T, Oikawa H (January 2004). "Cloning of a gene cluster responsible for the biosynthesis of diterpene aphidicolin, a specific inhibitor of DNA polymerase α". Bioscience, Biotechnology, and Biochemistry. 68 (1): 146–52. doi:10.1271/bbb.68.146. PMID 14745177.

[1]

[2]