Big Bone Cave formed in Mississippian Montegeale limestone originally on the western escarpment of the Cumberland Plateau. The cave formed prior to 5.7 ±1.09 Ma based in cosmogenic radionuclide dating of pebbles washed into the Muster Ground room.[5] Long passages formed during a long period of water table stability when the climate was warmer and sea levels were higher reducing erosion throughout the Mississippi drainage.[6] At the time, the Cumberland River flowed across the Highland Rim before the formation of the Nashville Basin. Global cooling 3.2-3.1 Ma triggered a 75–100-metre (246–328 ft) sea-level drop which increased erosion (incision) up the Mississippi River and led to the Cumberland River cutting into the Highland Rim and its headwaters, the Collins River, cutting into the Cumberland Plateau. The lower water table would have drained the water filling the caverns. Further erosion isolated Bone Cave Mountain from the Cumberland Plateau.
^Gulden, Bob (November 28, 2016). "USA LONGEST CAVES". NSS Geo2 Long & Deep Caves. Archived from the original on April 21, 2006. Retrieved May 29, 2017.{{cite web}}: CS1 maint: unfit URL (link)
Smyre, John L. and Zawislak, Ronald L., Big Bone and the Caves of Bone Cave Mountain, (2007) Rocky River Press, Rock Island Tennessee, ISBN978-0-9779471-0-2, ISBN0-9779471-0-6.
Matthews, Larry E. Big Bone Cave, (2006) National Speleological Society, ISBN1-879961-24-5.
Barr, Thomas C., Jr., Caves Of Tennessee, (1961) Bulletin 64 of the Tennessee Division of Geology, See pages 451-460.