The Interstate Railroad generally followed the valleys of the Guest River and Powell River, with only one summit, at Norton, between those two rivers.
The railroad began at the Clinchfield Railroad's Miller Yard, along the Clinch River between Dungannon and Carfax. It started off paralleling the Clinchfield to the northeast until its crossing of the Guest River (the line between Scott County and Wise County). The Interstate crossed the Guest River and split away to the north, running first along its east shore and then along its west shore and through the short Swede Tunnel. The Interstate crossed the Guest River again, where the river turned west, and continued north through another tunnel to Maytown.
Other branches left the mainline east of Norton (to Glamorgan and Dixiana), at Dorchester Junction (to Needmore), and east of Kelly View at Kent Junction (to Roaring Fork and Pardee). These branches all ran north alongside waterways; the one to Dixiana used the valley of the Guest River, and the one to Needmore ran along the Powell River.
Heritage unit
As a part of Norfolk Southern's 30th anniversary, the company painted 20 new locomotives into predecessor schemes. NS #8105, a GE ES44AC locomotive, was painted into the Interstate scheme.