Not under a commission of lieutenancy 20 November 1591 – 29 April 1617
In commission 30 April 1617 – 1 June 1622
30 April 1617 – 27 April 1620: Sir Robert Fowler, Sir Richard Wigmore, Sir Allen Apsley, Sir Richard Morrison, Sir Lewis Lewknor, Sir William Smith, Sir William Slingsby, Sir John Keyes, Sir John Brett, Clement Edmondes, George Calvert, Edward Fortett, Henry Spiller, Edmund Doubleday, Thomas Sanderson, Francis Mitchell
27 April 1620 – 1 June 1622: Sir Edward Sackville, Sir Edward Cecil, Sir John Suckling, Sir Edward Zouch, Sir Richard Morrison, Sir Allen Apsley, Sir Francis Darcy, Sir Lewis Lewknor, Sir Richard Wigmore, Sir Thomas Middleton, Sir William Cockain, Sir Clement Edmondes, Sir Henry Spiller
A deputy lieutenant of Middlesex is commissioned by the Lord Lieutenant of Middlesex. Deputy lieutenants support the work of the lord-lieutenant. There can be several deputy lieutenants at any time, depending on the population of the county. Their appointment does not terminate with the changing of the lord-lieutenant, but they usually retire at age 75.