Protein Z (PZ or PROZ), vitamin K-dependent protein Z, is a protein encoded in the human by the PROZgene.[5][6]
Protein Z is a member of the coagulation cascade, the group of blood proteins that leads to the formation of blood clots. It is a glycoprotein. Protein Z functions to inhibit blood coagulation by binding to an inhibitor.[7] It is a GLA domain protein and thus Vitamin K-dependent, and its functionality is therefore impaired in warfarin therapy.
The main role of Protein Z appears to be the degradation of Factor Xa. This is done by Protein Z-related protease inhibitor (ZPI), but the reaction is accelerated 1000-fold by the presence of Protein Z. Oddly, ZPI also degrades Factor XI, but this reaction does not require the presence of Protein Z. ZPI activated by Protein Z does not appear to happen because of its conformation, but proximity to each other. When Protein Z in bound to ZPI, it will bind to the same phospholipid surface as Factor Xa. This is what promotes the inhibition of Factor Xa.[8]
In some studies, deficiency states have been associated with a propensity to thrombosis. Others, however, link it to bleeding tendency; there is no clear explanation for this, as it acts physiologically as an inhibitor, and deficiency would logically have led to a predisposition for thrombosis.
It has four domains: a GLA-rich region, two EGF-like domains and a trypsin-like domain. It lacks the serine residue that would make it catalytically active as a serine protease.
History
Protein Z was first isolated in cattle blood by Christopher Prowse and Peter Esnouf in 1977,[9] and Broze & Miletich determined it in human plasma in 1984.[10] Protein Z found in humans was given the same name as the one found in cattle for a few reasons. When looking at these isolated proteins it was found that they both have similar molecular weight, a similar composition of amino acids, and a similar Amino Terminal sequences.[11] These similarities in molecular composition of the protein found in cattle and humans was great enough that it can be concluded they were the same protein. When Protein Z was first discovered, it was theorized to be a form of Factor X instead of its own individual protein. Research had to be done to isolate this protein to find out if it was a form of Factor X or not. To test this, Vitamin K dependents were removed from the sample by adsorption to barium citrate, then an ion exchange chromatography was performed. This process showed that there was no Factor X in the isolated protein. The purified Protein Z in this experiment was distinct from Factor X, proving it was a separate protein.[11]
Structure
Structural analysis of Protein Z will allow better understanding of its function. The Ramachandran plot for Protein Z indicates it will form alpha helices. The final structure, all alpha domain, was determined by x-ray diffraction. It consists of chain A and B, which are both helix-loop-helix motifs.PDB: 1LP1[12] The secondary structures of this protein are color coded in the image in the top left; pink represents the strands, yellow represents alpha helices and white is the coils.
Health
There are many reasons that Protein Z is important in health. In pregnancy it is vital that the protein is functioning correctly. It has been found that if it isn't functioning correctly, it can lead to fetal death or hypersensitive disorders in pregnancy. This happens because when the levels of this protein drop too low, it can lead to fetal growth restrictions.[13] Another possible effect is having a high sensitivity to this protein which could correlate with diabetes.[14] In women diagnosed with ovarian cancers, it was found the protein was inhibiting Factor Xa which happens because there is a lower regulation of this protein in cancer cells.[15]
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^Ichinose A, Takeya H, Espling E, Iwanaga S, Kisiel W, Davie EW (November 1990). "Amino acid sequence of human protein Z, a vitamin K-dependent plasma glycoprotein". Biochemical and Biophysical Research Communications. 172 (3): 1139–1144. doi:10.1016/0006-291X(90)91566-B. PMID2244898.
^Sejima H, Hayashi T, Deyashiki Y, Nishioka J, Suzuki K (September 1990). "Primary structure of vitamin K-dependent human protein Z". Biochemical and Biophysical Research Communications. 171 (2): 661–668. doi:10.1016/0006-291X(90)91197-Z. PMID2403355.
^Prowse CV, Esnouf MP (1977). "The isolation of a new warfarin-sensitive protein from bovine plasma". Biochemical Society Transactions. 5 (1): 255–256. doi:10.1042/bst0050255. PMID892175.
