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EcoRI

EcoRI
EcoRI Estrutura cristalográfica da endonuclease EcoRI
Indicadores
Pfam	PF02963
InterPro	IPR004221
SCOP	1na6

EcoRI (pronunciado "eco érre um") é uma enzima isolada a partir de cepas de Escherichia coli e faz parte do sistema de restrição-modificação.

Em biologia molecular é utilizada como uma enzima de restrição. Ela cria extremidades coesivas com saliências na extremidade 5' de moléculas de DNA. A sequência de ácido nucleico em que ocorrem os cortes é GAATTC, que tem como sequência complementar CTTAAG.^[1]^[2]

Esta enzima é utilizada em uma grande variedade de técnicas de genética molecular, incluindo a clonagem, triagem de DNA e supressão de segmentos de DNA in vitro. As enzimas de restrição como a EcoRI, que geram extremidades coesivas de DNA são muitas vezes utilizadas para cortar o DNA antes da reação de ligação. EcoRI pode exibir corte não-específico, dependendo das condições presentes na reação. Estas condições incluem baixa concentração de sal, concentração de glicerol elevada, quantidade excessiva de enzima presente na reação, pH elevado e contaminação por certos solventes orgânicos.^[3]

Referências

↑ Pingoud, A., Jeltsch, A. (2001). «Structure and function of type II restriction endonucleases». Nucl. Acids Res. 29 (18): 3705–3727. PMC 55916. PMID 11557805. doi:10.1093/nar/29.18.3705
↑ Kurpiewski, M. R., Engler, L. E., Wozniak, L. A., Kobylanska, A., Koziolkiewicz, M., Stec, W. J, Jen-Jacobson, L (2004). «Mechanisms of coupling between DNA recognition and catalysis in EcoRI endonucleases». Structure. 12: 1775–1788. PMID 15458627. doi:10.1016/j.str.2004.07.016
↑ «Cópia arquivada». Consultado em 8 de outubro de 2012. Arquivado do original em 15 de outubro de 2012

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