According to the promotion rules decided upon in 2019, the Regionalliga Südwest and West retained direct promotion spots. Based on a rotation principle, the Regionalliga Nordost received the third direct promotion spot this season, while the Regionalliga Bayern and Nord champions played a promotion play-off.[1]
^ abcdReserve teams are ineligible for qualification for the DFB-Pokal.
^Türkgücü München was deducted 2 points for fielding two players against 1. FC Nürnberg II without submitting proof of their social insurance within the deadline.[7]
^1. FC Schweinfurt was deducted 1 point due to riots during the away match against Würzburger Kickers.[8]
Ordinarily, the relegation play-offs are played as two-legged matches between the 15th and 16th-placed teams of the Regionalliga Bayern and the runners-up of the two Bayernliga divisions. However, since only one team from the 2023–24 Bayernliga Süd, Schwaben Augsburg, applied for a Regionalliga license, thereby gaining direct promotion, the three remaining teams played a single round-robin tournament instead, with the top two qualifying for the next Regionalliga season.[10]
The order of the legs was determined in a draw. The matches were scheduled for 22 and 26 May 2024, but since Würzburger Kickers qualified for the final of the 2023–24 Bavarian Cup, scheduled for 25 May 2024, the matches were instead played on 29 May and 2 June 2024.[12]