4-Hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il glukozidna beta-D-glukozidaza

4-Hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il glukozidna beta-D-glukozidaza
Identifikatori
EC broj	3.2.1.182
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

4-Hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il glukozidna beta-D-glukozidaza (EC 3.2.1.182, DIMBOAGlc hidrolaza, DIMBOA glukozidaza) je enzim sa sistematskim imenom (2R)-4-hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il beta-D-glukopiranozid beta-D-glukozidaza.^[1]^[2]^[3]^[4]^[5]^[6] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) (2R)-4-hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il beta-D-glukopiranozid + H₂O

\rightleftharpoons

2,4-dihidroksi-7-metoksi-2H-1,4-benzoksazin-3(4H)-on + D-glukoza

(2) (2R)-4-hidroksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il beta-D-glukopiranozid + H₂O

\rightleftharpoons

2,4-dihidroksi-2H-1,4-benzoksazin-3(4H)-on + D-glukoza

Enzim iz Triticum aestivum (pšenice) ima veći afinitet za DIMBOA glukozid nego za DIBOA glukozid.

Reference

↑ Sue, M., Ishihara, A. and Iwamura, H. (2000). „Purification and characterization of a hydroxamic acid glucoside β-glucosidase from wheat (Triticum aestivum L.) seedlings”. Planta 210: 432-438. PMID 10750901.
↑ Sue, M., Ishihara, A. and Iwamura, H. (2000). „Purification and characterization of a β-glucosidase from rye (Secale cereale L.) seedlings”. Plant Sci. 155: 67-74. PMID 10773341.
↑ Czjzek, M., Cicek, M., Zamboni, V., Bevan, D.R., Henrissat, B. and Esen, A. (2000). „The mechanism of substrate (aglycone) specificity in β-glucosidases is revealed by crystal structures of mutant maize β-glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes”. Proc. Natl. Acad. Sci. USA 97: 13555-13560. PMID 11106394.
↑ Nikus, J., Esen, A. and Jonsson, L.M.V. (2003). „Cloning of a plastidic rye (Secale cereale) β-glucosidase cDNA and its expression in Escherichia coli”. Physiol. Plantarum 118: 337-348.
↑ Sue, M., Yamazaki, K., Yajima, S., Nomura, T., Matsukawa, T., Iwamura, H. and Miyamoto, T. (2006). „Molecular and structural characterization of hexameric β-D-glucosidases in wheat and rye”. Plant Physiol. 141: 1237-1247. PMID 16751439.
↑ Sue, M., Nakamura, C., Miyamoto, T. and Yajima, S. (2011). „Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae”. Plant Sci. 180: 268-275. PMID 21421370.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Spoljašnje veze

MeSH 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl+glucoside+beta-D-glucosidase

[1] Sue, M., Ishihara, A. and Iwamura, H. (2000). „Purification and characterization of a hydroxamic acid glucoside β-glucosidase from wheat (Triticum aestivum L.) seedlings”. Planta 210: 432-438. PMID 10750901.

[2] Sue, M., Ishihara, A. and Iwamura, H. (2000). „Purification and characterization of a β-glucosidase from rye (Secale cereale L.) seedlings”. Plant Sci. 155: 67-74. PMID 10773341.

[3] Czjzek, M., Cicek, M., Zamboni, V., Bevan, D.R., Henrissat, B. and Esen, A. (2000). „The mechanism of substrate (aglycone) specificity in β-glucosidases is revealed by crystal structures of mutant maize β-glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes”. Proc. Natl. Acad. Sci. USA 97: 13555-13560. PMID 11106394.

[4] Nikus, J., Esen, A. and Jonsson, L.M.V. (2003). „Cloning of a plastidic rye (Secale cereale) β-glucosidase cDNA and its expression in Escherichia coli”. Physiol. Plantarum 118: 337-348.

[5] Sue, M., Yamazaki, K., Yajima, S., Nomura, T., Matsukawa, T., Iwamura, H. and Miyamoto, T. (2006). „Molecular and structural characterization of hexameric β-D-glucosidases in wheat and rye”. Plant Physiol. 141: 1237-1247. PMID 16751439.

[6] Sue, M., Nakamura, C., Miyamoto, T. and Yajima, S. (2011). „Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae”. Plant Sci. 180: 268-275. PMID 21421370.

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